Hotspot

lhbA-trnG-UCC

LSC · 37558-38311 · 754 bp

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Pi 0.0468

MarkerSeek score 75.20

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 33
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	TTTTCTGGCTCGGCTATTCC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1743-1755 bp	1.0	85.2
lhbA-trnG-UCC_p2	TTTTCTGGCTCGGCTATTCC	GGGGCGTATTCAATCCTTGT	50.0	50.0	58.0	57.9	1836-1847 bp	1.0	84.8
lhbA-trnG-UCC_p3	TTTTCTGGCTCGGCTATTCC	CCGAGAGCTAACTACCGAGA	50.0	55.0	58.0	58.0	2057-2058 bp	1.0	84.3
lhbA-trnG-UCC_p4	TTTTCTGGCTCGGCTATTCC	CGTCTCCGCAACATTGAAAC	50.0	50.0	58.0	58.0	1732 bp	0.5	59.3
lhbA-trnG-UCC_p5	CGAATAGGACACGGCCATAG	CTTGAGGTCACGGGTTCAAA	55.0	50.0	57.9	58.0	1522 bp	0.5	59.2