Hotspot

trnD-GUC-trnY-GUA

LSC · 31034-31464 · 431 bp

Back to Emblica

Pi 0.0565

MarkerSeek score 78.90

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 24
pi: 0.0565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATTTGGATCTGTCGGGAC	GGGGACGGACTGTAAATTCG	55.0	55.0	58.0	58.4	580-588 bp	1.0	80.9
trnD-GUC-trnY-GUA_p2	GGATTTGGATCTGTCGGGAC	GGGACGGACTGTAAATTCGT	55.0	50.0	58.0	57.6	579-587 bp	1.0	80.8
trnD-GUC-trnY-GUA_p3	GGATTTGGATCTGTCGGGAC	GATTCTTCCTGGGTCGATGC	55.0	55.0	58.0	58.4	613-621 bp	1.0	80.2
trnD-GUC-trnY-GUA_p4	GGATTTGGATCTGTCGGGAC	CGGACTGTAAATTCGTTGGC	55.0	50.0	58.0	57.5	575-583 bp	1.0	79.6
trnD-GUC-trnY-GUA_p5	TTTGGATCTGTCGGGACTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.4	58.4	577-585 bp	1.0	78.4