primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	161	366	273-328	1.000000	85.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	161	366	276-331	1.000000	85.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	161	366	287-342	1.000000	85.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	161	366	286-341	1.000000	85.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	161	366	288-343	1.000000	85.0
rps16_p1	rps16	1	TGGTTATAAATTATCCACACCCTCT	ACCGGTTCAAAATTCAAGGA	25	20	36.0	40.0	57.5	55.5	0.0	0.0	8.002616	5476	6643	1231-1231	0.500000	20.0
rps16_p2	rps16	2	TGGTTATAAATTATCCACACCCTCT	ACCGGTTCAAAATTCAAGGAA	25	21	36.0	38.1	57.5	56.3	0.0	0.0	8.201051	5476	6643	1231-1231	0.500000	20.0
rps16_p3	rps16	3	TGGTTATAAATTATCCACACCCTC	ACCGGTTCAAAATTCAAGGA	24	20	37.5	40.0	56.2	55.5	0.0	0.0	8.320010	5476	6643	1231-1231	0.500000	20.0
rps16_p4	rps16	4	TGGTTATAAATTATCCACACCCT	ACCGGTTCAAAATTCAAGGA	23	20	34.8	40.0	55.1	55.5	0.0	0.0	8.478211	5476	6643	1231-1231	0.500000	20.0
rps16_p5	rps16	5	TGGTTATAAATTATCCACACCCTC	ACCGGTTCAAAATTCAAGGAA	24	21	37.5	38.1	56.2	56.3	0.0	0.0	8.518445	5476	6643	1231-1231	0.500000	20.0
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10743	11153	453-491	1.000000	70.2
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10743	11153	456-494	1.000000	70.0
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10743	11153	459-497	1.000000	69.5
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10743	11153	462-500	1.000000	69.3
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10743	11153	457-495	1.000000	67.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14245	15374	1184-1217	1.000000	83.5
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGG	20	20	45.0	55.0	58.0	58.2	1.4	21.3	0.218634	14245	15374	1158-1191	1.000000	83.2
atpH-atpI_p3	atpH-atpI	3	CAGTACCTTGACCAACTCCG	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.213026	14245	15374	1243-1276	1.000000	82.9
atpH-atpI_p4	atpH-atpI	4	CAGTACCTTGACCAACTCCG	TAGGTGAATCCATGGAGGGG	20	20	55.0	55.0	57.9	58.2	0.0	21.3	0.260533	14245	15374	1217-1250	1.000000	82.7
atpH-atpI_p5	atpH-atpI	5	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.261511	14245	15374	1222-1255	1.000000	82.6
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGATTTGGATCTGTCGGGAC	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.396569	31034	31464	580-588	1.000000	80.9
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GGATTTGGATCTGTCGGGAC	GGGACGGACTGTAAATTCGT	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.420246	31034	31464	579-587	1.000000	80.8
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGATTTGGATCTGTCGGGAC	GATTCTTCCTGGGTCGATGC	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.457526	31034	31464	613-621	1.000000	80.2
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GGATTTGGATCTGTCGGGAC	CGGACTGTAAATTCGTTGGC	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.565759	31034	31464	575-583	1.000000	79.6
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	TTTGGATCTGTCGGGACTGA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.4	58.4	10.2	0.0	0.711774	31034	31464	577-585	1.000000	78.4
lhbA-trnG-UCC_p1	lhbA-trnG-UCC	1	TTTTCTGGCTCGGCTATTCC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.0	58.0	0.0	4.2	0.076593	37558	38311	1743-1755	1.000000	85.2
lhbA-trnG-UCC_p2	lhbA-trnG-UCC	2	TTTTCTGGCTCGGCTATTCC	GGGGCGTATTCAATCCTTGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.091169	37558	38311	1836-1847	1.000000	84.8
lhbA-trnG-UCC_p3	lhbA-trnG-UCC	3	TTTTCTGGCTCGGCTATTCC	CCGAGAGCTAACTACCGAGA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.087559	37558	38311	2057-2058	1.000000	84.3
lhbA-trnG-UCC_p4	lhbA-trnG-UCC	4	TTTTCTGGCTCGGCTATTCC	CGTCTCCGCAACATTGAAAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.087915	37558	38311	1732-1732	0.500000	59.3
lhbA-trnG-UCC_p5	lhbA-trnG-UCC	5	CGAATAGGACACGGCCATAG	CTTGAGGTCACGGGTTCAAA	20	20	55.0	50.0	57.9	58.0	2.2	4.2	0.104199	37558	38311	1522-1522	0.500000	59.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	CCGGAAAGTTGGATAGGGTG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.040892	49470	49974	652-653	1.000000	83.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	CGGAAAGTTGGATAGGGTGG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.040892	49470	49974	651-652	1.000000	83.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	49470	49974	616-617	1.000000	83.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	49470	49974	615-616	1.000000	83.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTGGTAGAGCAGAGGACTGA	CGGAAAGTTGGATAGGGTGG	20	20	55.0	55.0	57.9	58.0	2.5	0.0	0.174685	49470	49974	639-640	1.000000	82.6
psaI-ycf4_p1	psaI-ycf4	1	AGTATTTCCGGCAATTGCGA	CGAGACCCCGCTAGAAATTC	20	20	45.0	55.0	58.3	58.1	23.5	0.0	0.400541	61143	61542	514-517	1.000000	81.1
psaI-ycf4_p2	psaI-ycf4	2	AGTATTTCCGGCAATTGCGA	TCGAGACCCCGCTAGAAATT	20	20	45.0	50.0	58.3	58.5	23.5	0.0	0.772897	61143	61542	515-518	1.000000	78.2
psaI-ycf4_p3	psaI-ycf4	3	AGTATTTCCGGCAATTGCGA	TTCGAGACCCCGCTAGAAAT	20	20	45.0	50.0	58.3	58.5	23.5	0.0	0.772897	61143	61542	516-519	1.000000	78.1
psaI-ycf4_p4	psaI-ycf4	4	TATTTCCGGCAATTGCGATG	CGAGACCCCGCTAGAAATTC	20	20	45.0	55.0	57.2	58.1	23.5	0.0	0.918341	61143	61542	512-515	1.000000	77.0
psaI-ycf4_p5	psaI-ycf4	5	GTATTTCCGGCAATTGCGAT	CGAGACCCCGCTAGAAATTC	20	20	45.0	55.0	57.2	58.1	23.5	0.0	0.920212	61143	61542	513-516	1.000000	77.0
ycf4-cemA_p1	ycf4-cemA	1	GTCGCATCCTACTCCGATTC	CTTCCCTATCCACGATGCTG	20	20	55.0	55.0	57.9	57.8	0.0	0.0	0.214104	62092	63152	1886-1906	1.000000	82.2
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	CTTCCCTATCCACGATGCTG	20	20	45.0	55.0	58.1	57.8	3.3	0.0	0.256535	62092	63152	1710-1730	1.000000	82.2
ycf4-cemA_p3	ycf4-cemA	3	TGGGGTTTTTGTTGGTTGGA	CTTCCCTATCCACGATGCTG	20	20	45.0	55.0	58.0	57.8	0.0	0.0	0.208201	62092	63152	2129-2149	1.000000	81.9
ycf4-cemA_p4	ycf4-cemA	4	GTCGCATCCTACTCCGATTC	ACTTCCCTATCCACGATGCT	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.258568	62092	63152	1887-1907	1.000000	81.9
ycf4-cemA_p5	ycf4-cemA	5	TGGGGTTTTTGTTGGTTGGA	ACTTCCCTATCCACGATGCT	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.252664	62092	63152	2130-2150	1.000000	81.5
petA-psbJ_p1	petA-psbJ	1	TCGAGAAAGTTCAATTGTCCGA	ACTGGTATTCCTGTGATCGG	22	20	40.9	50.0	58.0	56.7	5.1	0.0	3.294652	64809	65721	1023-1206	1.000000	59.1
petA-psbJ_p2	petA-psbJ	2	TCGAGAAAGTTCAATTGTCCGA	CTGGTATTCCTGTGATCGGT	22	20	40.9	50.0	58.0	56.7	5.1	0.0	3.294652	64809	65721	1022-1205	1.000000	59.1
petA-psbJ_p3	petA-psbJ	3	TCGAGAAAGTTCAATTGTCCGA	ACTGGTATTCCTGTGATCGGT	22	21	40.9	47.6	58.0	58.5	5.1	0.0	3.547112	64809	65721	1023-1206	1.000000	57.1
petA-psbJ_p4	petA-psbJ	4	TCGAGAAAGTTCAATTGTCCGA	AACTGGTATTCCTGTGATCGG	22	21	40.9	47.6	58.0	57.5	5.1	1.6	3.558671	64809	65721	1024-1207	1.000000	57.0
petA-psbJ_p5	petA-psbJ	5	TCGAGAAAGTTCAATTGTCCGA	CTGGTATTCCTGTGATCGGTT	22	21	40.9	47.6	58.0	57.5	5.1	0.0	3.558671	64809	65721	1022-1205	1.000000	57.0
petL-petG_p1	petL-petG	1	GGTCTGACCAAGATACGACT	GCAGTTACGAATAATCCAGCC	20	21	50.0	47.6	56.4	57.1	21.7	0.0	3.498502	67271	67444	268-275	1.000000	56.4
petL-petG_p2	petL-petG	2	GGTCTGACCAAGATACGACTT	GCAGTTACGAATAATCCAGCC	21	21	47.6	47.6	57.1	57.1	21.7	0.0	3.750564	67271	67444	268-275	1.000000	54.4
petL-petG_p3	petL-petG	3	ACCTCAGCTCTGTTTATTGGTC	GCAGTTACGAATAATCCAGCC	22	21	45.5	47.6	58.1	57.1	0.0	0.0	3.935845	67271	67444	286-293	1.000000	52.7
petL-petG_p4	petL-petG	4	AGCTCTGTTTATTGGTCTGACC	GCAGTTACGAATAATCCAGCC	22	21	45.5	47.6	58.1	57.1	20.0	0.0	3.935845	67271	67444	281-288	1.000000	52.7
petL-petG_p5	petL-petG	5	ACCTCAGCTCTGTTTATTGGT	GCAGTTACGAATAATCCAGCC	21	21	42.9	47.6	56.9	57.1	0.0	0.0	3.957988	67271	67444	286-293	1.000000	52.5
ndhF-rpl32_p1	ndhF-rpl32	1	AGCGTAATTAAAGGTAAAATCCATGA	AGCTTTCAACGCTACCCAAT	26	20	30.8	45.0	57.5	57.8	0.0	0.0	6.714703	113544	114486	874-1083	1.000000	47.4
ndhF-rpl32_p2	ndhF-rpl32	2	AGCGTAATTAAAGGTAAAATCCATGA	AAAGCTTTCAACGCTACCCA	26	20	30.8	45.0	57.5	58.0	0.0	5.7	6.538670	113544	114486	876-1085	1.000000	47.3
ndhF-rpl32_p3	ndhF-rpl32	3	AGCGTAATTAAAGGTAAAATCCATGA	AAGCTTTCAACGCTACCCAA	26	20	30.8	45.0	57.5	58.0	0.0	0.0	6.538670	113544	114486	875-1084	1.000000	47.3
ndhF-rpl32_p4	ndhF-rpl32	4	AGCGTAATTAAAGGTAAAATCCA	AAAGCTTTCAACGCTACCCA	23	20	30.4	45.0	54.3	58.0	0.0	5.7	6.675244	113544	114486	876-1085	1.000000	47.3
ndhF-rpl32_p5	ndhF-rpl32	5	AGCGTAATTAAAGGTAAAATCCA	AAGCTTTCAACGCTACCCAA	23	20	30.4	45.0	54.3	58.0	0.0	0.0	6.675244	113544	114486	875-1084	1.000000	47.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CAAAAGGCTTTTTGTACGACA	AGCGTGTCTACCGATTTCAC	21	20	38.1	50.0	55.6	58.0	22.0	0.0	3.443745	114640	115696	1089-1112	1.000000	59.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CAAAAGGCTTTTTGTACGACA	CAGCGTGTCTACCGATTTCA	21	20	38.1	50.0	55.6	58.0	22.0	0.0	3.445116	114640	115696	1090-1113	1.000000	59.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CAAAAGGCTTTTTGTACGACA	TCCTAAGAGCAGCGTGTCTA	21	20	38.1	50.0	55.6	57.9	22.0	0.0	3.560192	114640	115696	1099-1122	1.000000	58.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CAAAAGGCTTTTTGTACGACA	CCTAAGAGCAGCGTGTCTAC	21	20	38.1	55.0	55.6	57.8	22.0	0.0	3.632210	114640	115696	1098-1121	1.000000	57.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CAAAAGGCTTTTTGTACGACA	CTAAGAGCAGCGTGTCTACC	21	20	38.1	55.0	55.6	57.8	22.0	0.0	3.632210	114640	115696	1097-1120	1.000000	57.6
ndhD-psaC_p1	ndhD-psaC	1	GAACCCGCGAATATCGGTAA	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	57.9	58.0	8.2	0.0	0.157340	118605	118710	212-212	1.000000	82.5
ndhD-psaC_p2	ndhD-psaC	2	GAACCCGCGAATATCGGTAA	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	57.9	58.0	8.2	0.0	0.169493	118605	118710	218-218	1.000000	82.3
ndhD-psaC_p3	ndhD-psaC	3	AAAGAACCCGCGAATATCGG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.4	58.0	8.2	0.0	0.440161	118605	118710	215-215	1.000000	80.2
ndhD-psaC_p4	ndhD-psaC	4	AAAGAACCCGCGAATATCGG	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.4	58.0	8.2	0.0	0.452314	118605	118710	221-221	1.000000	80.0
ndhD-psaC_p5	ndhD-psaC	5	GAACCCGCGAATATCGGTAA	TCGAAGCATGGGTCTAGGTT	20	20	50.0	50.0	57.9	58.4	8.2	0.0	0.570092	118605	118710	205-205	1.000000	79.3
ndhA_p1	ndhA	1	GCAGCTCGTATCGTTTGTTG	AGAGTTGAAGCACCAAAGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.158170	120979	123219	2549-2592	1.000000	82.7
ndhA_p2	ndhA	2	GCAGCTCGTATCGTTTGTTG	AACCCGCTGCTTTGATAGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.174891	120979	123219	2661-2704	1.000000	82.4
ndhA_p3	ndhA	3	ACTCAACACAGTTGCCACAA	AGAGTTGAAGCACCAAAGGG	20	20	45.0	50.0	58.2	58.0	5.1	0.0	0.180234	120979	123219	2830-2873	1.000000	82.4
ndhA_p4	ndhA	4	CGTGACGATCATACGTGGAA	AGAGTTGAAGCACCAAAGGG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.196022	120979	123219	2893-2936	1.000000	82.2
ndhA_p5	ndhA	5	TTCGTGACGATCATACGTGG	AGAGTTGAAGCACCAAAGGG	20	20	50.0	50.0	57.8	58.0	9.1	0.0	0.196022	120979	123219	2938-2938	0.500000	58.4
rps15-ycf1_p1	rps15-ycf1	1	TGAAATTCAACCGATCCCGT	TGCTAGTCGGTTCAGTATGG	20	20	45.0	50.0	57.5	56.8	0.0	0.0	1.731517	124783	125173	526-528	1.000000	71.0
rps15-ycf1_p2	rps15-ycf1	2	TGAAATTCAACCGATCCCGT	GCTAGTCGGTTCAGTATGGT	20	20	45.0	50.0	57.5	56.7	0.0	0.0	1.733724	124783	125173	525-527	1.000000	71.0
rps15-ycf1_p3	rps15-ycf1	3	TGAAATTCAACCGATCCCGT	TGCTAGTCGGTTCAGTATGGT	20	21	45.0	47.6	57.5	58.5	0.0	0.0	2.029494	124783	125173	526-528	1.000000	68.6
rps15-ycf1_p4	rps15-ycf1	4	TGAAATTCAACCGATCCCGT	ATGCTAGTCGGTTCAGTATGG	20	21	45.0	47.6	57.5	57.3	0.0	0.0	2.218251	124783	125173	527-529	1.000000	67.1
rps15-ycf1_p5	rps15-ycf1	5	TTGAAATTCAACCGATCCCGT	TGCTAGTCGGTTCAGTATGG	21	20	42.9	50.0	58.2	56.8	4.0	0.0	2.462941	124783	125173	527-529	1.000000	65.1
ycf1_p1	ycf1	1	CAGTTTCCATTCCCCCAACA	TAAGTGGGCCTTATCGTGGA	20	20	50.0	50.0	58.0	58.1	0.0	4.5	0.139530	128466	128585	1308-1320	1.000000	83.2
ycf1_p2	ycf1	2	CAGTTTCCATTCCCCCAACA	AGAAAACGTGGCAGAGGATC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.108847	128466	128585	845-845	0.500000	59.1
ycf1_p3	ycf1	3	GTTTCCATTCCCCCAACAGT	AGAAAACGTGGCAGAGGATC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.110286	128466	128585	843-843	0.500000	59.1
ycf1_p4	ycf1	4	CAGTTTCCATTCCCCCAACA	GAAGAAAACGTGGCAGAGGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.129289	128466	128585	847-847	0.500000	59.0
ycf1_p5	ycf1	5	GTTTCCATTCCCCCAACAGT	GAAGAAAACGTGGCAGAGGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.130728	128466	128585	845-845	0.500000	59.0