Hotspot

trnL-CAA-ycf2

IRa · 147675-148390 · 716 bp

Back to Embelia

Pi 0.00791

MarkerSeek score 63.90

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 716
variable_sites: 9
pi: 0.00791
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	GCTTTTCCCGGATGAAATGC	50.0	50.0	58.0	58.1	822-828 bp	1.0	80.2
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	GCTTTTCCCGGATGAAATGC	50.0	50.0	58.0	58.1	824-830 bp	1.0	80.2
trnL-CAA-ycf2_p3	GCCTTGGTGGTGAAATGGTA	GCTTTTCCCGGATGAAATGC	50.0	50.0	58.1	58.1	834-840 bp	1.0	79.6
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	825-831 bp	1.0	79.0
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	824-830 bp	1.0	79.0