Hotspot

psaC-ndhE

SSC · 120432-120714 · 283 bp

Back to Embelia

Pi 0.0154

MarkerSeek score 62.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 283
variable_sites: 8
pi: 0.0154
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GCCGTCCCAAGGTATCATTT	CTGAAGCAGCGATTGGATTG	50.0	50.0	57.9	57.5	474-483 bp	1.0	77.6
psaC-ndhE_p2	CAAGCTCGGACACATTGAGT	CTGAAGCAGCGATTGGATTG	50.0	50.0	58.2	57.5	437-446 bp	1.0	76.6
psaC-ndhE_p3	AATACATCTGTGGGGCAAGC	CTGAAGCAGCGATTGGATTG	50.0	50.0	58.2	57.5	452-461 bp	1.0	76.3
psaC-ndhE_p4	CGGACACATTGAGTACACCC	CTGAAGCAGCGATTGGATTG	55.0	50.0	58.3	57.5	431-440 bp	1.0	76.0
psaC-ndhE_p5	GCCGTCCCAAGGTATCATTT	GCAGCGATTGGATTGGCTAT	50.0	50.0	57.9	58.8	469-478 bp	1.0	75.4