Hotspot

rpl32-trnL-UAG

SSC · 116352-117138 · 787 bp

Back to Embelia

Pi 0.0178

MarkerSeek score 60.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 787
variable_sites: 24
pi: 0.0178
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAATAAGT	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	54.1	57.9	837-850 bp	1.0	51.2
rpl32-trnL-UAG_p2	TGTGCGACAAACAAATAAGT	AGCAGCGTGTCTACCAATTT	35.0	45.0	54.1	57.8	830-843 bp	1.0	50.7
rpl32-trnL-UAG_p3	TGTGCGACAAACAAATAAGT	CCTAAGAGCAGCGTGTCTAC	35.0	55.0	54.1	57.8	836-849 bp	1.0	50.6
rpl32-trnL-UAG_p4	TGTGCGACAAACAAATAAGT	CTAAGAGCAGCGTGTCTACC	35.0	55.0	54.1	57.8	835-848 bp	1.0	50.6
rpl32-trnL-UAG_p5	TGTGCGACAAACAAATAAGT	TTTTAGAAGAGGGCATGCCG	35.0	50.0	54.1	58.2	893-906 bp	1.0	49.9