Hotspot

ndhF-rpl32

SSC · 115275-116189 · 915 bp

Back to Embelia

Pi 0.0169

MarkerSeek score 56.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 915
variable_sites: 24
pi: 0.0169
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAAACGCCTTTTCACAAGC	GCCCGGTGGAAAGAGATTTA	45.0	50.0	58.1	57.6	1323-1583 bp	0.75	71.7
ndhF-rpl32_p2	CAAACGCCTTTTCACAAGCA	GCCCGGTGGAAAGAGATTTA	45.0	50.0	58.1	57.6	1322-1582 bp	0.75	71.7
ndhF-rpl32_p3	AAAAGCCCACATACGACGAA	GCCCGGTGGAAAGAGATTTA	45.0	50.0	57.8	57.6	910-1170 bp	0.75	71.4
ndhF-rpl32_p4	ACAAACGCCTTTTCACAAGC	AAACTTTTTGACTGCCCGGT	45.0	45.0	58.1	58.5	1336-1596 bp	0.75	70.8
ndhF-rpl32_p5	CAAACGCCTTTTCACAAGCA	AAACTTTTTGACTGCCCGGT	45.0	45.0	58.1	58.5	1335-1595 bp	0.75	70.8