Hotspot

ycf2-trnL-CAA

IRb · 96057-96772 · 716 bp

Back to Embelia

Pi 0.00791

MarkerSeek score 63.90

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 716
variable_sites: 9
pi: 0.00791
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	GCTTTTCCCGGATGAAATGC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.1	58.0	822-828 bp	1.0	80.2
ycf2-trnL-CAA_p2	GCTTTTCCCGGATGAAATGC	TGAAATGGTAGACACGCGAG	50.0	50.0	58.1	58.0	824-830 bp	1.0	80.2
ycf2-trnL-CAA_p3	GCTTTTCCCGGATGAAATGC	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.1	58.1	834-840 bp	1.0	79.6
ycf2-trnL-CAA_p4	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	825-831 bp	1.0	79.0
ycf2-trnL-CAA_p5	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	824-830 bp	1.0	79.0