Hotspot

accD-psaI

LSC · 60489-61204 · 716 bp

Back to Embelia

Pi 0.0156

MarkerSeek score 60.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 18
pi: 0.0156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	45.0	45.0	57.4	57.9	849-880 bp	1.0	77.1
accD-psaI_p2	ACTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	45.0	45.0	57.4	57.9	848-879 bp	1.0	77.1
accD-psaI_p3	ACTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	45.0	45.0	57.4	58.2	838-869 bp	1.0	76.8
accD-psaI_p4	CAACTCCACGCTTTCTTTCC	AAAGAAGCCATTGCCATTGC	50.0	45.0	57.3	57.9	850-881 bp	1.0	76.7
accD-psaI_p5	AACTCCACGCTTTCTTTCCT	TTGCCATTGCCGGAAATACT	45.0	45.0	57.4	58.2	839-870 bp	1.0	76.7