Hotspot

rbcL-accD

LSC · 58359-58994 · 636 bp

Back to Embelia

Pi 0.0162

MarkerSeek score 61.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 15
pi: 0.0162
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ATCAATAGGACCAAGGCTGC	50.0	50.0	57.9	57.6	779-792 bp	1.0	79.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	AGGCTGCCCATTGATTTACT	50.0	45.0	57.9	57.2	766-779 bp	1.0	75.9
rbcL-accD_p3	CTGCTGCTTGTGAGGTATGG	ATCAATAGGACCAAGGCTGC	55.0	50.0	58.6	57.6	781-794 bp	1.0	75.6
rbcL-accD_p4	TGCTTGTGAGGTATGGAAGG	ATCAATAGGACCAAGGCTGC	50.0	50.0	57.2	57.6	777-790 bp	1.0	74.3
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	CCAAGGCTGCCCATTGATTT	50.0	50.0	57.9	59.1	769-782 bp	1.0	73.9