Hotspot

psbZ-trnG-GCC

LSC · 37507-37766 · 260 bp

Back to Embelia

Pi 0.031

MarkerSeek score 63.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 14
pi: 0.031
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TGGATTAGTTTTTCTGGTGGGT	CGTCTTCTCCTTGGCAAAGA	40.9	50.0	57.5	57.8	351-357 bp	1.0	62.5
psbZ-trnG-GCC_p2	TTGGATTAGTTTTTCTGGTGGGT	CGTCTTCTCCTTGGCAAAGA	39.1	50.0	58.2	57.8	352-358 bp	1.0	56.7
psbZ-trnG-GCC_p3	TGGATTAGTTTTTCTGGTGGG	CGTCTTCTCCTTGGCAAAGA	42.9	50.0	55.8	57.8	351-357 bp	1.0	56.3
psbZ-trnG-GCC_p4	GGATTAGTTTTTCTGGTGGGT	CGTCTTCTCCTTGGCAAAGA	42.9	50.0	55.8	57.8	350-356 bp	1.0	56.3
psbZ-trnG-GCC_p5	TGGATTAGTTTTTCTGGTGGGT	CGGATAGCGGGAATCGAAC	40.9	57.9	57.5	58.2	373-379 bp	1.0	54.2