Hotspot

trnE-UUC-trnT-GGU

LSC · 31798-32671 · 874 bp

Back to Embelia

Pi 0.0135

MarkerSeek score 62.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 21
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1015-1036 bp	1.0	80.8
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	994-1015 bp	1.0	80.4
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	991-1012 bp	1.0	80.2
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	1012-1033 bp	1.0	79.6
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TTGAACCGATGACTTACGCC	55.0	50.0	57.8	58.3	1017-1038 bp	1.0	78.6