Hotspot

ycf3

LSC · 43219-45202 · 1984 bp

Back to Elytrophorus

Pi 0.00353

MarkerSeek score 59.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1984
variable_sites: 7
pi: 0.00353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GGATCAACAACCAAACTACCC	TCCATTTAGAGCATATGGATCGA	47.6	39.1	56.8	57.0	2130 bp	1.0	40.3
ycf3_p2	GGATCAACAACCAAACTACCCA	TCCATTTAGAGCATATGGATCGA	45.5	39.1	58.5	57.0	2130 bp	1.0	40.3
ycf3_p3	TGGATCAACAACCAAACTACCC	TCCATTTAGAGCATATGGATCGA	45.5	39.1	58.5	57.0	2131 bp	1.0	40.3
ycf3_p4	GGATCAACAACCAAACTACCC	CCATTTAGAGCATATGGATCGA	47.6	40.9	56.8	55.6	2129 bp	1.0	40.3
ycf3_p5	GGATCAACAACCAAACTACCC	TCCATTTAGAGCATATGGATCG	47.6	40.9	56.8	55.6	2130 bp	1.0	40.3