Hotspot

trnV-UAC

LSC · 52572-52610 · 39 bp

Back to Elytrophorus

Pi 0.0256

MarkerSeek score 60.90

Primer available yes

Label trnV-UAC

Feature Metadata

label_name: trnV-UAC
feature_type: tRNA
region: LSC
length_bp: 39
variable_sites: 1
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC_p1	TGTCAGGCTACTGTTCTCCT	AGGGATATCCTCCGGCATTA	50.0	50.0	57.7	57.4	224 bp	1.0	72.7
trnV-UAC_p2	TGTCAGGCTACTGTTCTCCT	GGGATATCCTCCGGCATTAG	50.0	55.0	57.7	57.0	223 bp	1.0	70.0
trnV-UAC_p3	TTCCCTTGAAAAGCATTGGC	AGGGATATCCTCCGGCATTA	45.0	50.0	57.2	57.4	135 bp	1.0	68.8
trnV-UAC_p4	CCCTTGAAAAGCATTGGCG	AGGGATATCCTCCGGCATTA	52.6	50.0	57.9	57.4	133 bp	1.0	66.5
trnV-UAC_p5	TTGTCAGGCTACTGTTCTCC	AGGGATATCCTCCGGCATTA	50.0	50.0	56.9	57.4	225 bp	1.0	66.2