Hotspot

ndhA

SSC · 111821-113934 · 2114 bp

Back to Elytrophorus

Pi 0.00568

MarkerSeek score 60.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2114
variable_sites: 12
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTATCATAGGGAACA	TATGGGGGAAGTCGATCGTT	42.9	50.0	55.7	58.2	2248-2252 bp	1.0	52.6
ndhA_p2	ACCCAGTTATCATAGGGAACA	TTATGGGGGAAGTCGATCGT	42.9	50.0	55.7	58.2	2249-2253 bp	1.0	52.6
ndhA_p3	GAACCCAGTTATCATAGGGAACA	TATGGGGGAAGTCGATCGTT	43.5	50.0	57.6	58.2	2250-2254 bp	1.0	51.8
ndhA_p4	GAACCCAGTTATCATAGGGAACA	TTATGGGGGAAGTCGATCGT	43.5	50.0	57.6	58.2	2251-2255 bp	1.0	51.8
ndhA_p5	TGAACCCAGTTATCATAGGGAAC	TATGGGGGAAGTCGATCGTT	43.5	50.0	57.6	58.2	2251-2255 bp	1.0	51.8