Hotspot

rps3-rpl22

LSC · 80733-80789 · 57 bp

Back to Elytrophorus

Pi 0.0175

MarkerSeek score 62.60

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 1
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.9	57.9	227-228 bp	1.0	78.9
rps3-rpl22_p2	GTGCGAACCAAAAGGAATGG	AAAAATTCAGACCTCGGGCT	50.0	45.0	57.9	57.4	228-229 bp	1.0	75.1
rps3-rpl22_p3	GTTGTGCGAACCAAAAGGAA	AAAATTCAGACCTCGGGCTC	45.0	50.0	57.4	57.9	230-231 bp	1.0	74.9
rps3-rpl22_p4	TGTGCGAACCAAAAGGAATG	AAAATTCAGACCTCGGGCTC	45.0	50.0	57.2	57.9	228-229 bp	1.0	73.1
rps3-rpl22_p5	TCTTCCTGTAGACCGTCAGA	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.1	57.9	261-262 bp	1.0	72.4