Hotspot

trnR-UCU-rps14

LSC · 37236-37617 · 382 bp

Back to Elytrophorus

Pi 0.0105

MarkerSeek score 60.80

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 4
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	ATGCATGTTTGTTACCGGGT	47.6	45.0	56.9	58.1	457-459 bp	1.0	64.0
trnR-UCU-rps14_p2	AGAAGACCTCTGTCCTATCCA	TCCGGACACATACTTCGAGA	47.6	50.0	56.9	57.9	485-487 bp	1.0	63.6
trnR-UCU-rps14_p3	AGGTTTAGAAGACCTCTGTCCT	ATGCATGTTTGTTACCGGGT	45.5	45.0	57.9	58.1	463-465 bp	1.0	63.3
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	446-448 bp	1.0	63.2
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	TCCGGACACATACTTCGAGA	45.5	50.0	57.9	57.9	491-493 bp	1.0	62.9