Hotspot

rpoB-rpoC1

LSC · 23746-23782 · 37 bp

Back to Elytrophorus

Pi 0.027

MarkerSeek score 68.00

Primer available yes

Label rpoB-rpoC1

Feature Metadata

label_name: rpoB-rpoC1
feature_type: igs
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-rpoC1_p1	GCTCGTTCGAGAACTACGAT	CCAACGACTTCCCCATTAGG	50.0	55.0	57.5	58.0	232 bp	1.0	76.4
rpoB-rpoC1_p2	GCTCGTTCGAGAACTACGAT	CCTTGTGACTTCACCAACGA	50.0	50.0	57.5	58.1	245 bp	1.0	76.3
rpoB-rpoC1_p3	AGTGCTCGTTCGAGAACTAC	CCAACGACTTCCCCATTAGG	50.0	55.0	57.4	58.0	235 bp	1.0	75.1
rpoB-rpoC1_p4	AGTGCTCGTTCGAGAACTAC	CCTTGTGACTTCACCAACGA	50.0	50.0	57.4	58.1	248 bp	1.0	75.0
rpoB-rpoC1_p5	GCTCGTTCGAGAACTACGAT	TGACTTCACCAACGACTTCC	50.0	50.0	57.5	57.8	240 bp	1.0	74.8