Hotspot

trnC-GCA-rpoB

LSC · 19519-20517 · 999 bp

Back to Elytrophorus

Pi 0.002

MarkerSeek score 59.90

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 2
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1083 bp	1.0	77.0
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1082 bp	1.0	77.0
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TCAAGTTCTTCTGCCAAGCC	50.0	50.0	58.1	58.4	1149 bp	1.0	76.4
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1066 bp	1.0	76.3
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CTTCTGCCAAGCCCTGATTA	50.0	50.0	58.1	57.6	1142 bp	1.0	76.2