Hotspot

rps16

LSC · 4511-5580 · 1070 bp

Back to Elymus

Pi 0.00159
MarkerSeek score 49.50
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1070
variable_sites
11
pi
0.00159
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TGGCACGATATAAATCCAAATAGG TGCTCCATAGATAGCAAGTT 37.5 40.0 56.9 53.8 1287-1290 bp 1.0 40.9
rps16_p2 TGGCACGATATAAATCCAAATAGG TCCATATTGCTCCATAGATAGCA 37.5 39.1 56.9 56.7 1294-1297 bp 1.0 40.9
rps16_p3 TGGCACGATATAAATCCAAATAGG TGCTCCATAGATAGCAAGTTT 37.5 38.1 56.9 54.7 1287-1290 bp 1.0 40.9
rps16_p4 TGGCACGATATAAATCCAAA TGCTCCATAGATAGCAAGTT 35.0 40.0 52.6 53.8 1287-1290 bp 1.0 40.9
rps16_p5 TGGCACGATATAAATCCAAATAGG TGCTCCATAGATAGCAAGTTTT 37.5 36.4 56.9 55.5 1287-1290 bp 1.0 40.9