Hotspot

rpoC2

LSC · 24798-29237 · 4440 bp

Back to Elymus

Pi 0.00119
MarkerSeek score 51.60
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4440
variable_sites
33
pi
0.00119
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 TCCAAGGCGAAGATTGAATCA TGAAGTACTTGGAAGGTTTGT 42.9 38.1 57.7 55.0 323 bp 1.0 41.2
rpoC2_p2 TCCAAGGCGAAGATTGAATCA AGTTCTTGAAGTACTTGGAAGGT 42.9 39.1 57.7 57.4 329 bp 0.956522 39.1
rpoC2_p3 CCAAGGCGAAGATTGAATCAC AGTTCTTGAAGTACTTGGAAGGT 47.6 39.1 57.6 57.4 328 bp 0.956522 39.0
rpoC2_p4 TCCAAGGCGAAGATTGAATCA AAGTTCTTGAAGTACTTGGAAGGT 42.9 37.5 57.7 58.0 330 bp 0.956522 39.0
rpoC2_p5 TCCAAGGCGAAGATTGAATCA AGTTCTTGAAGTACTTGGAAGGTT 42.9 37.5 57.7 58.0 329 bp 0.956522 39.0