Hotspot

rpl16

LSC · 77737-79198 · 1462 bp

Back to Elymus

Pi 0.00167
MarkerSeek score 52.20
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1462
variable_sites
12
pi
0.00167
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCCAGAAAGAAAAACCGGGC TCCTGAAATCCCTGCAAAAGA 50.0 42.9 58.7 57.5 1596-1606 bp 1.0 63.6
rpl16_p2 CCAGAAAGAAAAACCGGGCA TCCTGAAATCCCTGCAAAAGA 50.0 42.9 59.0 57.5 1595-1605 bp 1.0 61.3
rpl16_p3 CAGAAAGAAAAACCGGGCAT TCCTGAAATCCCTGCAAAAGA 45.0 42.9 56.9 57.5 1594-1604 bp 1.0 60.1
rpl16_p4 TCCAGAAAGAAAAACCGGGC CCTGAAATCCCTGCAAAAGA 50.0 45.0 58.7 55.9 1595-1605 bp 1.0 59.0
rpl16_p5 TCCAGAAAGAAAAACCGGGC TCCTGAAATCCCTGCAAAAG 50.0 45.0 58.7 55.9 1596-1606 bp 1.0 59.0