Hotspot

rpl32-trnL-UAG

SSC · 104802-105503 · 702 bp

Back to Elymus

Pi 0.00602
MarkerSeek score 57.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
702
variable_sites
25
pi
0.00602
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTCCAGCGGTAATGAGCATC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.0 58.0 839-842 bp 1.0 82.3
rpl32-trnL-UAG_p2 TTCCAGCGGTAATGAGCATC GGATTCGAACCGAGATGCTT 50.0 50.0 58.0 58.1 842-845 bp 1.0 81.8
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 811-814 bp 1.0 81.7
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 810-813 bp 1.0 81.7
rpl32-trnL-UAG_p5 TTCCAGCGGTAATGAGCATC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 812-815 bp 1.0 81.4