Hotspot

rbcL-psaI

LSC · 55816-57461 · 1646 bp

Back to Elymus

Pi 0.00535
MarkerSeek score 58.90
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1646
variable_sites
33
pi
0.00535
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 CGCAGCTTGTGAAGTATGGA GTCATTGCAATTGCCGGAAA 50.0 45.0 58.3 57.9 1777-1801 bp 1.0 79.0
rbcL-psaI_p2 AGTTCGAGCCGGTAGATACT GTCATTGCAATTGCCGGAAA 50.0 45.0 57.7 57.9 1742-1766 bp 1.0 78.5
rbcL-psaI_p3 GGCGATCAAATTCGAGTTCG GTCATTGCAATTGCCGGAAA 50.0 45.0 57.6 57.9 1756-1780 bp 1.0 78.4
rbcL-psaI_p4 TGGAAGGCGATCAAATTCGA GTCATTGCAATTGCCGGAAA 45.0 45.0 57.6 57.9 1761-1785 bp 1.0 78.0
rbcL-psaI_p5 AGCTTGCAAATGGAGTCCTG GTCATTGCAATTGCCGGAAA 50.0 45.0 58.5 57.9 1804-1828 bp 1.0 77.5