Hotspot

ccsA

SSC · 108409-109374 · 966 bp

Back to Elymandra

Pi 0.0069

MarkerSeek score 49.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 10
pi: 0.0069
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGGTTTCGGACTGGTACATC	GGGAATGAGCGGTTTTGTTG	55.0	50.0	58.0	57.9	1838-1845 bp	1.0	80.3
ccsA_p2	CTCAAGCATCTCGGTTCGAA	GGGAATGAGCGGTTTTGTTG	50.0	50.0	58.0	57.9	1589 bp	1.0	80.2
ccsA_p3	AAGCATCTCGGTTCGAATCC	GGGAATGAGCGGTTTTGTTG	50.0	50.0	58.1	57.9	1586 bp	1.0	79.7
ccsA_p4	ATTAGCCGCCATGGTGAAAT	GGGAATGAGCGGTTTTGTTG	45.0	50.0	57.9	57.9	1639 bp	1.0	79.6
ccsA_p5	CTCAAGCATCTCGGTTCGAA	GAATGAGCGGTTTTGTTGCA	50.0	45.0	58.0	57.9	1587 bp	1.0	79.6