Hotspot

psaC-ndhE

SSC · 111423-111875 · 453 bp

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Pi 0.0118

MarkerSeek score 63.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 453
variable_sites: 8
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCTGTTGGGCAAGCTC	TTTTGTTATAGCCCTTGCGG	50.0	45.0	57.9	56.4	643-658 bp	1.0	67.2
psaC-ndhE_p2	TACATCTGTTGGGCAAGCTC	CTTCCATCCATCGTAACAGGA	50.0	47.6	57.9	57.2	590-605 bp	1.0	65.6
psaC-ndhE_p3	TACATCTGTTGGGCAAGCTC	TCTTCCATCCATCGTAACAGG	50.0	47.6	57.9	57.2	591-606 bp	1.0	65.6
psaC-ndhE_p4	TACATCTGTTGGGCAAGCTC	TTTTTGTTATAGCCCTTGCGG	50.0	42.9	57.9	57.1	644-659 bp	1.0	65.2
psaC-ndhE_p5	GGCAAGCTCGTACACATTGA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.6	56.4	632-647 bp	1.0	63.7