Hotspot

rpl20-rps12

LSC · 68027-68715 · 689 bp

Back to Elymandra

Pi 0.00677

MarkerSeek score 63.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 7
pi: 0.00677
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	CGACATGCCAACGGTTAAAC	45.0	50.0	58.0	58.0	861-862 bp	1.0	80.2
rpl20-rps12_p2	GTTTGAGGCAAATGAACGCA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.9	58.0	876-877 bp	1.0	79.2
rpl20-rps12_p3	ACGCATTTTTGTTCGTCGTC	TGCAAGACAGCCAATACGAA	45.0	45.0	58.0	57.8	828-829 bp	1.0	79.2
rpl20-rps12_p4	TTTTTGTTCGTCGTCTCCGA	CGACATGCCAACGGTTAAAC	45.0	50.0	57.8	58.0	856-857 bp	1.0	78.7
rpl20-rps12_p5	ACGCATTTTTGTTCGTCGTC	GAACATGCGCTAGGGTGTAT	45.0	50.0	58.0	57.8	763-764 bp	1.0	78.7