Hotspot

rpl33-rps18

LSC · 66684-66974 · 291 bp

Back to Elymandra

Pi 0.0162

MarkerSeek score 50.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 7
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GAAAGGAGGTTCATGGCCAA	GGAATCAACGAGCATGTTGC	50.0	50.0	58.1	58.1	1106-1159 bp	1.0	80.4
rpl33-rps18_p2	AAAGGAGGTTCATGGCCAAG	GGAATCAACGAGCATGTTGC	50.0	50.0	58.1	58.1	1105-1158 bp	1.0	80.3
rpl33-rps18_p3	AAAGGAGGTTCATGGCCAAG	CGCCCACGAAAAGTTTGTTT	50.0	45.0	58.1	58.1	619-647 bp	0.666667	67.1
rpl33-rps18_p4	GAAAGGAGGTTCATGGCCAA	CGCCCACGAAAAGTTTGTTT	50.0	45.0	58.1	58.1	620-648 bp	0.666667	67.0
rpl33-rps18_p5	TTTGGGGCTTTAGCAGGTTT	CGCCCACGAAAAGTTTGTTT	45.0	45.0	57.9	58.1	1050-1081 bp	0.666667	67.0