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psbE-petL

LSC · 63537-64825 · 1289 bp

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Pi 0.0042

MarkerSeek score 58.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 8
pi: 0.0042
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCA	45.0	45.0	58.0	57.3	1430-1447 bp	1.0	76.1
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.0	57.3	1429-1446 bp	1.0	76.1
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1419-1436 bp	1.0	75.8
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1418-1435 bp	1.0	75.8
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	58.2	57.3	1420-1437 bp	1.0	74.4