Hotspot

psbF-psbE

LSC · 63275-63284 · 10 bp

Back to Elymandra

Pi 0.0667

MarkerSeek score 75.00

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 10
variable_sites: 1
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GTGGGTACAGCTAATCCGTG	GTTTGGAAGTCCTCGACCAA	55.0	50.0	58.1	57.8	196 bp	1.0	77.9
psbF-psbE_p2	GTGGGTACAGCTAATCCGTG	TTGGAAGTCCTCGACCAAAC	55.0	50.0	58.1	57.8	194 bp	1.0	77.9
psbF-psbE_p3	GTGGGTACAGCTAATCCGTG	AAAGCCGACAAGGAATTCCA	55.0	45.0	58.1	57.7	161 bp	1.0	77.7
psbF-psbE_p4	TGGGTACAGCTAATCCGTGA	GTTTGGAAGTCCTCGACCAA	50.0	50.0	58.1	57.8	195 bp	1.0	77.3
psbF-psbE_p5	TGGGTACAGCTAATCCGTGA	TTGGAAGTCCTCGACCAAAC	50.0	50.0	58.1	57.8	193 bp	1.0	77.3