Hotspot

trnC-GCA-rpoB

LSC · 19573-20778 · 1206 bp

Back to Elymandra

Pi 0.00774

MarkerSeek score 56.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1206
variable_sites: 14
pi: 0.00774
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1286-1309 bp	1.0	78.6
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1285-1308 bp	1.0	78.6
trnC-GCA-rpoB_p3	GATTTTTGATCAGGCGGCAC	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1325-1348 bp	1.0	78.5
trnC-GCA-rpoB_p4	GATTTTTGATCAGGCGGCAC	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1324-1347 bp	1.0	78.5
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1269-1292 bp	1.0	78.0