Hotspot

psbM-petN

LSC · 18326-18941 · 616 bp

Back to Elymandra

Pi 0.0101

MarkerSeek score 54.20

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 9
pi: 0.0101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	CTTTAGACCCCCAAAAGCGT	GAAAGATAGAAAGCGGGGCA	50.0	50.0	58.1	58.0	1752-1790 bp	1.0	81.2
psbM-petN_p2	CTTTAGACCCCCAAAAGCGT	AAAGATAGAAAGCGGGGCAG	50.0	50.0	58.1	58.0	1751-1789 bp	1.0	81.2
psbM-petN_p3	CTTTAGACCCCCAAAAGCGT	TCTCTATTCCCGCGTCTTCT	50.0	50.0	58.1	57.9	1597-1635 bp	1.0	81.2
psbM-petN_p4	CTTTAGACCCCCAAAAGCGT	TCTCTCTATTCCCGCGTCTT	50.0	50.0	58.1	57.9	1599-1637 bp	1.0	81.2
psbM-petN_p5	CTTTAGACCCCCAAAAGCGT	GCAGCAGTAGGGTTTAGGTC	50.0	55.0	58.1	58.0	1752-1773 bp	0.666667	67.6