Hotspot

trnG-UCC-trnT-GGU

LSC · 13916-15926 · 2011 bp

Back to Elymandra

Pi 0.00715
MarkerSeek score 53.60
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
2011
variable_sites
21
pi
0.00715
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CTTCTGTCTTACCAAGGCCA 38.1 50.0 55.5 57.1 2111-2158 bp 1.0 47.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT ACTTCTGTCTTACCAAGGCC 38.1 50.0 55.5 57.1 2112-2159 bp 1.0 47.7
trnG-UCC-trnT-GGU_p3 AGAACGAATCACACTTTTACCAC CTTCTGTCTTACCAAGGCCA 39.1 50.0 57.3 57.1 2114-2161 bp 1.0 46.6
trnG-UCC-trnT-GGU_p4 GAACGAATCACACTTTTACCACT CTTCTGTCTTACCAAGGCCA 39.1 50.0 57.3 57.1 2113-2160 bp 1.0 46.6
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ACTTCTGTCTTACCAAGGCC 39.1 50.0 57.3 57.1 2114-2161 bp 1.0 46.6