Hotspot

ycf1

SSC · 121334-126826 · 5493 bp

Back to Elsholtzia

Pi 0.0425

MarkerSeek score 75.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5493
variable_sites: 1037
pi: 0.0425
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCGCATTTGCTTGAAACAA	CCTCCCGGTTTAGCCTTTTT	40.0	50.0	58.1	58.1	965 bp	0.25	56.6
ycf1_p2	GCAAGTGGAATGCTATCCCA	CCTCCCGGTTTAGCCTTTTT	50.0	50.0	57.9	58.1	1190 bp	0.25	55.9
ycf1_p3	GCAATTGGAGTATACGGCCA	CCTCCCGGTTTAGCCTTTTT	50.0	50.0	58.0	58.1	1297 bp	0.21875	52.4
ycf1_p4	TGCAATTGGAGTATACGGCC	CCTCCCGGTTTAGCCTTTTT	50.0	50.0	58.0	58.1	1298 bp	0.21875	52.4
ycf1_p5	GGACCAAAAGGAAGTTCCGT	CCTCCCGGTTTAGCCTTTTT	50.0	50.0	58.0	58.1	1571 bp	0.0625	45.2