Hotspot

petN-psbM

LSC · 27770-28769 · 1000 bp

Back to Elsholtzia

Pi 0.0398

MarkerSeek score 77.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 181
pi: 0.0398
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	CCCTTATTTCCCCGGGATTG	50.0	55.0	58.0	58.0	1250-1806 bp	1.0	91.9
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	CCCTTATTTCCCCGGGATTG	50.0	55.0	58.0	58.0	1248-1804 bp	1.0	91.9
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTACCGACCGCT	50.0	50.0	58.0	58.0	593-1154 bp	1.0	91.6
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTACCGACCGCT	50.0	50.0	58.0	58.0	591-1152 bp	1.0	91.6
petN-psbM_p5	TCCGGGAAAAATTGCATCCA	CCCTTATTTCCCCGGGATTG	45.0	55.0	58.1	58.0	1576-2132 bp	0.90625	87.7