Hotspot

rpoB-trnC-GCA

LSC · 26303-27446 · 1144 bp

Back to Elsholtzia

Pi 0.0333

MarkerSeek score 74.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 174
pi: 0.0333
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	ACTTCTTCCCCACACTACGA	50.0	50.0	58.0	58.0	1833-2010 bp	0.75	81.4
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	TTCTTCCCCACACTACGAGT	50.0	50.0	58.0	58.0	1831-2008 bp	0.75	81.4
rpoB-trnC-GCA_p3	TTGTTTCCTACTCACGCGAG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	1847-2024 bp	0.75	81.1
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.0	58.0	2025-2202 bp	0.75	80.9
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.0	58.0	2023-2200 bp	0.75	80.9