Hotspot

rps16-trnQ-UUG

LSC · 5948-6763 · 816 bp

Back to Elsholtzia

Pi 0.0501

MarkerSeek score 84.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 186
pi: 0.0501
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	769-1075 bp	1.0	90.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.1	57.9	782-1088 bp	1.0	89.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCTCGCTATT	45.0	50.0	58.1	58.3	791-1004 bp	0.90625	85.0
rps16-trnQ-UUG_p4	AAAAATCCACAACGGGTCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	796-1009 bp	0.875	83.9
rps16-trnQ-UUG_p5	AAAAATCCACAACGGGTCGT	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.3	57.9	809-1022 bp	0.875	83.5