Hotspot

petB

LSC · 77646-79084 · 1439 bp

Back to Eleutherococcus

Pi 0.00713

MarkerSeek score 41.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1439
variable_sites: 26
pi: 0.00713
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGTTGAGAAGGTTGCCCAAA	ACTTTCTGGGTCGTCTCAGA	45.0	50.0	57.8	58.0	2636-2647 bp	1.0	80.5
petB_p2	TTGTTGAGAAGGTTGCCCAA	ACTTTCTGGGTCGTCTCAGA	45.0	50.0	57.8	58.0	2637-2648 bp	1.0	80.5
petB_p3	GTGTATTTCTAGCCCAGCCC	TTCAAGCGAACCAGCCTATC	55.0	50.0	58.0	58.0	1829-1841 bp	0.769231	72.6
petB_p4	GTGTATTTCTAGCCCAGCCC	ACTTTCTGGGTCGTCTCAGA	55.0	50.0	58.0	58.0	2156-2168 bp	0.769231	72.5
petB_p5	GTGTATTTCTAGCCCAGCCC	CTTCAAGCGAACCAGCCTAT	55.0	50.0	58.0	58.0	1830-1842 bp	0.769231	72.5