Hotspot

clpP

LSC · 72691-74694 · 2004 bp

Back to Eleutherococcus

Pi 0.00425

MarkerSeek score 35.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2004
variable_sites: 35
pi: 0.00425
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	AACAGGGTCAGAGGGATCAA	50.0	50.0	58.0	58.0	2867-2905 bp	1.0	81.1
clpP_p2	CTGAACGAGTCGCACATACA	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2866-2904 bp	1.0	81.1
clpP_p3	CTGAACGAGTCGCACATACA	GATTCGGTATAGCCCCTCCT	50.0	55.0	58.0	58.1	2976-2999 bp	0.538462	61.5
clpP_p4	AGGGGGCCTAATCTTTCTGT	AATACGCAATGGGGAGGTTC	50.0	50.0	58.0	57.9	2781 bp	0.076923	42.6
clpP_p5	CTGAACGAGTCGCACATACA	AATACGCAATGGGGAGGTTC	50.0	50.0	58.0	57.9	2512 bp	0.076923	42.5