Hotspot

ndhG-ndhI

SSC · 121826-122205 · 380 bp

Back to Eleutherococcus

Pi 0.0121

MarkerSeek score 44.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 380
variable_sites: 15
pi: 0.0121
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCAGCAGCTACAAAATGG	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1026-1034 bp	1.0	81.9
ndhG-ndhI_p2	GTGCAGCAGCTACAAAATGG	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1025-1033 bp	1.0	81.9
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	899-907 bp	1.0	81.9
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	898-906 bp	1.0	81.9
ndhG-ndhI_p5	AATACCACTCCCAGACCTCC	ACAAACAGTACGAGCTGCAA	55.0	45.0	58.1	58.1	924-932 bp	1.0	81.2