Hotspot

rpl32-trnL (UAG)

SSC · 116483-117309 · 827 bp

Back to Eleutherococcus

Pi 0.00836
MarkerSeek score 52.50
Primer available yes
Label rpl32-trnL (UAG)

Feature Metadata

label_name
rpl32-trnL (UAG)
feature_type
igs
region
SSC
length_bp
827
variable_sites
23
pi
0.00836
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL (UAG)_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 927-962 bp 1.0 83.6
rpl32-trnL (UAG)_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 928-963 bp 1.0 83.6
rpl32-trnL (UAG)_p3 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 937-972 bp 1.0 82.6
rpl32-trnL (UAG)_p4 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 916-951 bp 1.0 82.5
rpl32-trnL (UAG)_p5 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 917-952 bp 1.0 82.5