Hotspot

ndhF-rpl32

SSC · 115121-116317 · 1197 bp

Back to Eleutherococcus

Pi 0.00846

MarkerSeek score 49.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1197
variable_sites: 33
pi: 0.00846
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CAAAGCGCGTTCCATTTCAT	50.0	45.0	58.0	58.0	1544-1607 bp	1.0	83.6
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	CCCCACCCCAAAATGAGAAA	50.0	50.0	58.0	58.0	1667-1729 bp	1.0	83.6
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	CAAAGCGCGTTCCATTTCAT	45.0	45.0	58.0	58.0	1542-1605 bp	1.0	83.5
ndhF-rpl32_p4	TTGCCGTCGGAAAAAGAAGA	CAAAGCGCGTTCCATTTCAT	45.0	45.0	58.0	58.0	1543-1606 bp	1.0	83.5
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	CCCCACCCCAAAATGAGAAA	45.0	50.0	58.0	58.0	1665-1727 bp	1.0	83.5