Hotspot

psaJ-rpl33

LSC · 69879-70326 · 448 bp

Back to Eleutherococcus

Pi 0.00872

MarkerSeek score 50.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 13
pi: 0.00872
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	ACCGTTTCGAACACAACTGA	45.0	45.0	58.0	58.0	542-580 bp	1.0	82.7
psaJ-rpl33_p2	ATGCGTTGACATTCCCCTTT	ACCGTTTCGAACACAACTGA	45.0	45.0	58.1	58.0	533-571 bp	1.0	82.4
psaJ-rpl33_p3	TTTTCCCAGATGCGTTGACA	CACCGTTTCGAACACAACTG	45.0	50.0	58.0	57.9	543-581 bp	1.0	82.2
psaJ-rpl33_p4	ATGCGTTGACATTCCCCTTT	CACCGTTTCGAACACAACTG	45.0	50.0	58.1	57.9	534-572 bp	1.0	81.8
psaJ-rpl33_p5	TTCCCAGATGCGTTGACATT	ACCGTTTCGAACACAACTGA	45.0	45.0	57.8	58.0	540-578 bp	1.0	81.4