Hotspot

accD-psaI

LSC · 61649-62337 · 689 bp

Back to Eleutherococcus

Pi 0.00785

MarkerSeek score 58.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 17
pi: 0.00785
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	793-817 bp	1.0	77.7
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	50.0	45.0	58.8	58.2	783-807 bp	1.0	77.4
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	TAAAGGCACCAACACAGAGG	50.0	50.0	58.8	57.7	755-779 bp	1.0	76.6
accD-psaI_p4	GCTCCACGCTTTCTTTCCTT	CACCAACACAGAGGGAAAGT	50.0	50.0	58.8	57.7	749-773 bp	1.0	76.0
accD-psaI_p5	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	52.6	45.0	58.1	57.9	793-817 bp	1.0	75.3