Hotspot

trnT (UGU)-trnL (UAA)

LSC · 49277-50101 · 825 bp

Back to Eleutherococcus

Pi 0.00837
MarkerSeek score 58.60
Primer available yes
Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name
trnT (UGU)-trnL (UAA)
feature_type
igs
region
LSC
length_bp
825
variable_sites
25
pi
0.00837
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT (UGU)-trnL (UAA)_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 813-875 bp 1.0 85.3
trnT (UGU)-trnL (UAA)_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 821-883 bp 1.0 84.7
trnT (UGU)-trnL (UAA)_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 845-907 bp 1.0 84.3
trnT (UGU)-trnL (UAA)_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 853-915 bp 1.0 83.8
trnT (UGU)-trnL (UAA)_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 827-889 bp 1.0 83.3