Hotspot

trnE (UUC)-trnT (GGU)

LSC · 33057-33887 · 831 bp

Back to Eleutherococcus

Pi 0.00445

MarkerSeek score 52.70

Primer available yes

Label trnE (UUC)-trnT (GGU)

Feature Metadata

label_name: trnE (UUC)-trnT (GGU)
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 11
pi: 0.00445
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE (UUC)-trnT (GGU)_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	910-935 bp	1.0	82.9
trnE (UUC)-trnT (GGU)_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	947-972 bp	1.0	82.8
trnE (UUC)-trnT (GGU)_p3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	875-900 bp	1.0	82.3
trnE (UUC)-trnT (GGU)_p4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	912-937 bp	1.0	82.2
trnE (UUC)-trnT (GGU)_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	888-913 bp	1.0	82.1