Hotspot

trnC (GCA)-petN

LSC · 29697-30417 · 721 bp

Back to Eleutherococcus

Pi 0.00493

MarkerSeek score 56.90

Primer available yes

Label trnC (GCA)-petN

Feature Metadata

label_name: trnC (GCA)-petN
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 13
pi: 0.00493
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC (GCA)-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	748-784 bp	1.0	84.7
trnC (GCA)-petN_p2	CGATATGGCCGAGTGGTAAG	TTAAAGCAGCCCAAGCAAGA	55.0	45.0	57.9	58.0	788-824 bp	1.0	84.5
trnC (GCA)-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	742-778 bp	1.0	83.2
trnC (GCA)-petN_p4	CGATATGGCCGAGTGGTAAG	CAGCCCAAGCAAGACTTACT	55.0	50.0	57.9	57.8	782-818 bp	1.0	83.1
trnC (GCA)-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	766-802 bp	1.0	82.3