Hotspot

atpH-atpI

LSC · 14942-16069 · 1128 bp

Back to Eleutherococcus

Pi 0.00759

MarkerSeek score 59.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1128
variable_sites: 31
pi: 0.00759
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1211-1232 bp	1.0	78.0
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1267-1288 bp	1.0	77.3
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1227-1248 bp	1.0	77.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1212-1233 bp	1.0	76.8
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	1271-1292 bp	1.0	76.7