Hotspot

trnG (UCC)-trnR (UCU)

LSC · 11120-11293 · 174 bp

Back to Eleutherococcus

Pi 0.0138

MarkerSeek score 51.90

Primer available yes

Label trnG (UCC)-trnR (UCU)

Feature Metadata

label_name: trnG (UCC)-trnR (UCU)
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 9
pi: 0.0138
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG (UCC)-trnR (UCU)_p1	AGCCTTCCAAGCTAACGATG	TCTTTCAAAAGGGCTTCCGA	50.0	45.0	58.0	57.4	330-361 bp	1.0	78.8
trnG (UCC)-trnR (UCU)_p2	CCTAGCCTTCCAAGCTAACG	TCTTTCAAAAGGGCTTCCGA	55.0	45.0	58.1	57.4	333-364 bp	1.0	78.5
trnG (UCC)-trnR (UCU)_p3	CCCTAGCCTTCCAAGCTAAC	TCTTTCAAAAGGGCTTCCGA	55.0	45.0	57.7	57.4	334-365 bp	1.0	76.3
trnG (UCC)-trnR (UCU)_p4	CTAACGATGCGGGTTCGATT	TCTTTCAAAAGGGCTTCCGA	50.0	45.0	58.4	57.4	319-350 bp	1.0	75.7
trnG (UCC)-trnR (UCU)_p5	TAACGATGCGGGTTCGATTC	TCTTTCAAAAGGGCTTCCGA	50.0	45.0	58.4	57.4	318-349 bp	1.0	75.7