Hotspot

rps16-trnQ (UUG)

LSC · 6482-8253 · 1772 bp

Back to Eleutherococcus

Pi 0.00548

MarkerSeek score 49.60

Primer available yes

Label rps16-trnQ (UUG)

Feature Metadata

label_name: rps16-trnQ (UUG)
feature_type: igs
region: LSC
length_bp: 1772
variable_sites: 36
pi: 0.00548
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ (UUG)_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1834-1857 bp	1.0	80.8
rps16-trnQ (UUG)_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1849-1872 bp	1.0	78.9
rps16-trnQ (UUG)_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1848-1871 bp	1.0	78.9
rps16-trnQ (UUG)_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1838-1861 bp	1.0	76.0
rps16-trnQ (UUG)_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1864-1887 bp	1.0	75.9